研究人员利用比较基因组学，鉴定出菌株 B31 中的一个特定基因，这有助于设计精确的引物和 Taqman 探针。由此建立的实时荧光定量聚合酶链式反应（PCR）检测方法对菌株 B31 具有高度特异性和高灵敏度，基因组的检测限为 28 拷贝 /μL。与常规 PCR 方法的对比分析 ...

PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...

What is the Put Call Ratio? The PCR is a common technical indicator used to measure the traded volume for put options in comparison with call options. The usage of PCR facilitates the analysis of ...

然后，运用 Sanger 测序对相关基因多态性进行检测；利用定量逆转录聚合酶链反应（qRT-PCR）测量 HLA-DQA 1 和 HLA-DQB 1 mRNA 的表达水平；通过流式细胞术检测淋巴细胞上 HLA-DQA 1 和 HLA-DQB 1 的表达；借助 TaqMan 荧光探针技术对 HLA-DQA 1 （rs1047989）进行基因分型并检测 ...

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