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生物通
15 天
综述:荧光原位杂交引导的单细胞拉曼激活分选测序(FISH-scRACS-seq ...
证明了 CARD-FISH 的高灵敏度和特异性 。对模拟菌群进行分选实验,结果表明,通过 16S PCR 产物的 Sanger 测序,所有 MDA 阳性细胞均为大肠杆菌特异性 16S rDNA 序列,SAG 的基因组完整性在 19.12% - 99.76% 之间,平均映射率为 35.21% - 47.53%,成功率在 40% - 70% 之间 ,空液滴 ...
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