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生物通
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基于FRET和bret的阿片受体构象传感器揭示激动剂诱发的阿片受体激活 ...
检测方法 单细胞 FRET 测量:在配备特定物镜、光源和相机的倒置显微镜下进行。用 435 nm 光激发 CFP,同时收集 CFP 和 YFP 荧光发射。细胞用外部缓冲液或激动剂溶液持续灌流,测量在室温下进行,数据采集借助 NIS - Elements 高级研究成像软件。 酶标仪多细胞 FRET ...
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Trans troops ban blocked
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Retires after 15 seasons
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