Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

Pellet 106 cells by centrifugation (250 - 300 x g) and carefully remove supernatant Make up 1X of fixation buffer by adding 5 ml of Cytofix (BD554655) to 10 ml of DPBS Add either 300μl (for microwell ...

Centrifuge at 500g at 4 °C for 5 min. 2|Resuspend the cell pellet in 0.5× volume of the original growth medium and measure the cell number using a hemocytometer. 4| Transfer 400-μl aliquots of ...

Here we introduce a new approach for the screening, selection and sorting of cell-surface–binding peptides from phage libraries. Biopanning and rapid analysis of selective interactive ligands ...