The development and use of biosensors based on fluorescence resonance energy transfer (FRET) has allowed researchers to achieve real-time visualization of metabolite behavior or signal ...
FRET is based on the dipole-dipole coupling mechanism, where the energy transfer occurs through non-radiative means. It is highly efficient over short distances and sensitive to the separation between ...
HomoFRET-FP, a less known FRET approach suitable for HTS aggregation studies in living cells Förster resonance energy transfer (FRET) is a popular tool in biochemistry and molecular cell biology. Most ...
During photobleaching FRET experiments, any increase in the CFP channel could thus arise from either FRET, or YFP to CFP photoconversion.
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