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FITC-脂多糖(LPS)|FITC-LPS在脓毒症免疫机制研究中的动态可视化应用
FITC-LPS可借助其荧光共振能量转移(FRET)特性,实现分子互作的可视化。 · 探针设计:采用铜催化叠氮-炔环加成(CuAAC)反应,将FITC定点-于LPS的脂质A疏水末端。 · 细胞模型:构建TLR4-GFP转基因巨噬细胞系,通过全内反射荧光显微镜(TIRFM)观察FITC-LPS与受体 ...
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