To convert a piece of cloned DNA into a centromere-containing human artificial chromosome (HAC), an array of repeated LacO sequences is incorporated into the DNA. The DNA is then transfected into ...

Kuroiwa et al. prepared a human artificial chromosome (HAC) vector containing the entire unrearranged sequences of the human immunoglobulin (hIg) heavy-chain (H) and lambda (λ) light-chain loci. They ...

The researchers also found that the unique sequences and structure of ... that enlarging the artificial chromosome DNA vector allowed for efficient formation of human artificial chromosomes ...