These merged fragments were subsequently cloned into the backbone that had been digested with XbaI, which contained the T7 RNA polymerase promoter and terminator. After that, the RSV pre - F antigen, ...

Attribution (BY): Credit must be given to the creator. The study of “resource allocator” bacteriophage T7 RNA polymerase (T7RNAP) has garnered significant interest, particularly for optimizing ...

However, they do not demonstrate the cloning of short fragments, such as gRNA sequences downstream of a U6 promoter, which would be an important proof-of-concept to validate these applications.

“Because the binding pocket of the RBP matches the sequence motif, the RBP binds to the specific set of several nucleotides. Then the RNA binding protein communicates to the splicing machinery to ...

Hyperactivation of RNA polymerase I (Pol I) transcription of ribosomal RNA (rRNA ... are described as either transcriptionally competent but non-transcribed or actively transcribed, depending on the ...

We have performed ChIP-seq experiments on tightly staged Drosophila embryos and show that massive recruitment of RNA polymerase II (Pol II) with widespread pausing occurs ... This global change in Pol ...