2019年4月29日 · NLRP3 (NOD-, LRR- and pyrin domain-containing protein 3) is an intracellular sensor that detects a broad range of microbial motifs, endogenous danger signals and environmental irritants,...

The NLRP3 inflammasome is a critical component of the innate immune system that mediates caspase-1 activation and the secretion of proinflammatory cytokines IL-1β/IL-18 in response to microbial infection and cellular damage.

2024年7月1日 · Inflammasomes are significant contributors to innate immunity, and one of the most extensively studied inflammasome complexes is NOD-like receptor 3 (NLRP3). NLRP3 has a wide range of...

NLRP3 inflammasome detects danger signals such as crystalline uric acid and extracellular ATP released by damaged cells. These signals release HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex.

2019年7月6日 · The NLRP3 inflammasome is a critical component of the innate immune system that mediates caspase-1 activation and the secretion of proinflammatory cytokines IL-1β/IL-18 in response to microbial infection and cellular damage.

The NLRP3 inflammasome is a multiprotein complex that plays a pivotal role in regulating the innate immune system and inflammatory signaling. Upon activation by PAMPs and DAMPs, NLRP3 oligomerizes and activates caspase-1 which initiates the ...

2021年7月28日 · In this review, we summarize the inextricable link between NLRP3 inflammasome activation and cell death and discuss potential therapeutics that target cell death effectors in NLRP3...

2022年6月10日 · The NLRP3 inflammasome is a multiprotein complex that plays a pivotal role in regulating the innate immune system and inflammatory signaling. Upon activation by PAMPs and DAMPs, NLRP3 oligomerizes and activates caspase-1 which initiates the processing and release of pro-inflammatory cytokines IL-1β …

2023年6月27日 · The NOD-like receptor protein 3 (NLRP3) inflammasome is a protein complex that regulates innate immune responses by activating caspase-1 and the inflammatory cytokines interleukin (IL)-1β and IL-18.

2022年11月3日 · The NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3) inflammasome is a cytoplasmic supramolecular complex that is activated in response to cellular perturbations triggered by infection and sterile injury.

Activation of NLRP3 leads to the formation of a cytosolic multiprotein signaling complex called the inflammasome, which serves as a platform for caspase-1 activation leading to the processing of proinflammatory cytokines IL-1β, IL-18 and GSDMD mediated cell death.

Inflammasomes are significant contributors to innate immunity, and one of the most extensively studied inflammasome complexes is NOD-like receptor 3 (NLRP3). NLRP3 has a wide range of recognition mechanisms that streamline immune activation and eliminate pathogens.

NLRP3 inflammasome participates in cardiovascular diseases. The NLRP3 inflammasome is a protein complex that can be activated by crystal or granular PAMPs and ischemic hypoxia risk-related molecular patterns, DAMPs, which promote the secretion of IL-1β and IL-18.

6 天之前 · The NLRP3 inflammasome plays a key role in the microglial innate immune response and, upon activation, leads to the caspase-1-dependent secretion of inflammatory cytokines such as IL-1β. 1 We have previously shown that the NLRP3 inflammasome has an important role in the progression of Alzheimer’s disease (AD). It is activated in the post-mortem brain tissue of AD …

2024年4月19日 · The NLRP3 inflammasome is a multiprotein signaling complex composed of NLRP3, a member of the NLR family, apoptosis-associated speck-like protein containing CARD (ASC), and caspase-1 ().The NLRP3 inflammasome is formed when NLRP3 recognizes a wide variety of damage-associated molecular patterns or pathogen associated molecular patterns …

2025年1月6日 · NLRP3 inflammasome activation causes autotomy and activation of the cysteine protease caspase1, which promotes maturation and secretion of IL-1β and IL-18, and induces pyroptosis. NLRP3 has been implicated in a wide range of pathological sterile inflammation associated with several autoinflammatory, ...

The NLRP3 inflammasome composed of the NLRP3 protein, procaspase-1, and ASC plays a vital role in regulating inflammation. In this review, NLRP3 regulation and activation, its proinflammatory role in inflammatory diseases, interactions with autophagy, and targeted therapeutic approaches in inflammatory diseases will be summarized.

NLRP3 inflammasome functions as a molecular platform, and triggers the activation of caspase-1 and cleavage of pro-IL-1β, pro-IL-18, and gasdermin D (GSDMD). Cleaved NT-GSDMD forms pores in the cell membrane and initiates pyroptosis, inducing cell death and release of many intracellular pro-inflammatory molecules.

2025年1月27日 · Changes in the levels of astrocytes and microglia are associated with the activation of the NLRP3 inflammasome and increased levels of proinflammatory cytokines, such as IL1β and TNFα. Interestingly, microglia-specific ABCA7ko restored Aβ42 peptide levels and IBA1+ and GFAP+ and NLRP3-related gene expression to the original APPPS1 mouse levels.

5 天之前 · The effect of SCFAs on PD may be mediated by NLRP3 inflammasome, and inhibiting NLRP3 may offer therapeutic benefits, induced by SCFAs, including diminished neuroinflammation, improved motor functions, and reduced α-syn deposition. Moreover, GPR43, identified as a critical SCFA receptor, also played a significant role in this mechanism.

2 天之前 · NLRP3 inflammasome activation has been reported in a variety of renal cells including podocytes . In fact, recent evidence supports immune cell-like functions of podocytes, ...

NLRP3, also known as cryopyrin, CIAS1, or NALP3, was initially described for its genetic association with forms of hereditary autoinflammation known as familial cold autoinflam-mation syndrome (FCAS), Muckle–Wells syndrome (MWS), and neonatal-onset multisystem inflammatory disease/chronic infantile neurologic cutaneous articular syndrome (NOMID/...

Distinctiveness of the nucleotide-binding oligomerization (NOD), Leucine-rich repeat (LRR)-containing protein (NLRP3) inflammasome resides in the diversity of molecules that induce its activation, indicating a certain intricacy.

4 天之前 · The NLRP3 inflammasome was quantified through qRT-PCR and using a GoTaq® qPCR Master kit (Promega, Madison, WI, USA). The NLRP3 primers were Forward – 5′CCATCAATGCTGCTTCGACA3 ′-; Reverse – 5′GAGCTCAGAACCAATGCGAG3 ′-. The qRT-PCR cycles were (1) 50 °C for 120 s, (2) 95 °C for 120 s, (3) 40 cycles of 95 °C for 15 s …

Inflammasome activation involves priming-dependent stimulation of NLRP3 expression, followed by NLRP3 assembly promoted by a cell danger signal, such as K + efflux or ATP. To minimize the effects of HNE on inflammasome priming, macrophages were treated with LPS for 3 hours before exposure to HNE and then stimulated with nigericin or ATP [ 37 ].

2020年2月17日 · The NLRP3 inflammasome composed of the NLRP3 protein, procaspase-1, and ASC plays a vital role in regulating inflammation. In this review, NLRP3 regulation and activation, its proinflammatory role in inflammatory diseases, interactions with autophagy, and targeted therapeutic approaches in inflammatory diseases will be summarized.

We describe the development of MCC950, a potent, selective, small-molecule inhibitor of NLRP3. MCC950 blocked canonical and noncanonical NLRP3 activation at nanomolar concentrations. MCC950 specifically inhibited activation of NLRP3 but not the AIM2, NLRC4 or NLRP1 inflammasomes.

The expression of NLRP3 inflammasome pathway-associated genes is increased in endothelial cells derived from induced pluripotent stem cells of patients with ICI myocarditis. 22 The expression of NLRP3 and related genes is significantly increased in human cardiomyocytes cocultured with lymphocytes and ICIs, as well as in the heart of mice ...

2019年9月7日 · NLRP3 is considered to be a sensor of microbial and other danger signals and plays a crucial role in mucosal immune responses, promoting the maturation of proinflammatory cytokines interleukin 1β (IL-1β) and IL-18.

2 天之前 · Dendritic cells (DCs) are specialized antigen-presenting cells that are present at low abundance in the circulation and tissues; they serve as crucial immune sentinels by continually sampling ...

IBA-1, CD68, and iNOS positive microglia and NLRP3 activation-related protein expression were also detected. SIRT3 was overexpressed in oxygen glucose deprivation (OGD)-induced microglia model, where cell morphology and expressions of pro-inflammatory cytokines and NLRP3 inflammasome activation-related proteins were examined.