2023年7月12日 · Structured illumination microscopy (SIM) has become the standard for next-generation wide-field microscopy, offering ultrahigh imaging speed, superresolution, a large field-of-view, and...

SIM - Pros and Cons. Structure illumination microscopy (SIM) allows: 2x increase in spatial resolution over wide-field microscopy. In 2D/3D-SIM, lateral only or both lateral and axial resolutions are improved, with respectively ~100 nm in …

2023年1月26日 · Three-dimensional structured illumination microscopy (3D SIM 1) excites the sample with non-uniform illumination, providing information outside the diffraction-limited passband that is...

2023年2月8日 · Thanks to advances in fluorescence microscopy, a type of light microscopy in which one wavelength of light is absorbed and another omitted, scientists can now use super-resolution microscopy to directly observe living subcellular structures and activities.

Structured illumination microscopy (SIM) reaches superresolution through collecting precise spatial features from systemic interaction with fixed lighting sequences.

2018年11月26日 · Structured illumination microscopy (SIM) allows rapid, super-resolution (SR) imaging in live specimens. We review recent technical advances in SR-SIM, with emphasis on imaging speed,...

2017年11月10日 · Super-resolved structured illumination microscopy (SR-SIM) is among the most rapidly growing fluorescence microscopy techniques that can surpass the optical diffraction limit.

Find Molecular Probes® fluorescence labels for the generation of structured illumination microscopy (SIM) images, useful for thicker sections and for 3D and live-cell imaging.

2021年11月19日 · This article first describes the principle and basic concept of general structured illumination microscopy (SIM) as a robust technique for optical super-resolution imaging. Then several types of SIM have been presented one by one and finally concluded with future possibilities and opportunities.

Structured Illumination Microscopy (SIM) and the high-speed alternative instant SIM (iSIM), can halve the resolution limit of conventional light microscopy. However, unlike other super-resolution methods, iSIM can capture images at up to 100Hz, and with a greater degree of optical sectioning (rejection of out-of-focus light).