From your description, it appears that the contamination isn't due to airborne microorganisms settling on top of the agar, but that it is present in the liquid agar at the time of pouring the...

agar plate. Freshly prepared agar plates are wet, therefore allow plates to dry during 1 to 3 days of incubation at room temperature. The dried LB plates can be stored at 4 °C or used freshly. Please note that at least 24 agar plates will be needed to test a single nanomaterial in 4 different

It might be your E. coli glycerol stock is cotaminated. Try to check it under microscope. One should appreciate your work, being the first time. There does not seem to be any problem in tubes or...

2016年9月21日 · In the first pic you can see the water droplets on lids and in the second pic you can see condensation on the walls of the plates. That condensation later drips down on agar and then I have water on my agar that drifts spores and mycelium all over. Proper pasteurization for guaranteed substrate.

1999年2月24日 · If some of your agar plates become contaminated, you can often tell by examining the plate how contamination took place. If the contaminants are imbedded in the agar, the contaminant was probably poured with the medium.

When condensation occurs within an agar plate, it’s usually visible as tiny droplets of water on the lid; sometimes the droplets are visible within the packaged sleeve of plates. But why does this condensation occur? Is condensation in agar plates to be expected? And does this impact the quality and performance of the prepared media?

In order to determine whether water has been contaminated by fecal material, a series of tests are used to demonstrate the presence or absence of coliforms. The coliform group is comprised of Gram-negative, nonspore-forming, aerobic to facultatively …

2019年9月4日 · Bacterial and fungal spores in the air and on surfaces can make freshly poured plates unusable, or can obscure results on inoculated plates. Being able to recognize these contaminants before using a compromised plate can save valuable time.

Sporadic agar plate contamination can occur, but usually this is not a frequent problem. One possible cause could be poor sterilization of the medium before pouring the plates. In this case, you...

2024年5月31日 · When working with agar in mycology, spotting contamination early is key. Think of contamination as the enemy of your mycelium; it competes for resources and can halt your project in its tracks. Common culprits include mold, bacteria, and yeasts, showing up as unwanted colors and fuzzy textures that aren’t your mycelium.