Immunohistochemistry (IHC) revealed qualitatively lower levels of MMP13 (Fig. 3a) and Cathepsin K (CatK, Fig. 3c) protein expression in subchondral bone of the medial OA tibial plateau...
了解详细信息：Immunohistochemistry (IHC) revealed qualitatively lower levels of MMP13 (Fig. 3a) and Cathepsin K (CatK, Fig. 3c) protein expression in subchondral bone of the medial OA tibial plateau...
www.nature.com/articles/s41413-019-0070-yAs the number of antibodies continues to grow, this technique has become vital for research laboratories studying the skeleton. Here we describe a detailed protocol for IHC analysis of bone and cartilage, addressing specific issues associated with staining of hard and matrix-rich tissues.
pubmed.ncbi.nlm.nih.gov/25331045/Mice with inactivation of one allele of the Col2a1 gene showed softer articular cartilage, disturbed collagenous network, reduced subchondral bone volume, and increased susceptibility to OA,...
www.nature.com/articles/s41413-019-0046-yImmunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a chromogen (immunohistochemistry or IHC) or ...
pmc.ncbi.nlm.nih.gov/articles/PMC8365415/Immunohistochemistry (IHC) staining revealed a positive correlation between the ratio of P16 and P21 positive cells and increased OARSI scores, indicating the coexistence of cellular senescence and exacerbated facet joint degeneration in mice (Fig. 1K – N). These findings collectively suggest an association between facet joint degeneration ...
www.sciencedirect.com/science/article/pii/S152994…Osteocyte dysfunction promotes osteoarthritis through MMP13
2019年11月5日 · Immunohistochemistry (IHC) revealed qualitatively lower levels of MMP13 (Fig. 3a) and Cathepsin K (CatK, Fig. 3c) protein expression in subchondral bone of the medial OA tibial plateau...
- 作者: Courtney M. Mazur, Jonathon J. Woo, Cristal S. Yee, Aaron J. Fields, Claire Acevedo, Claire Acevedo,...
- Publish Year: 2019
Immunohistochemistry of skeletal tissues - PubMed
As the number of antibodies continues to grow, this technique has become vital for research laboratories studying the skeleton. Here we describe a detailed protocol for IHC analysis of …
- 作者: Crystal Idleburg, Elizabeth N. DeLassus, Deborah V. Novack
- Publish Year: 2015
Collagen type II suppresses articular chondrocyte …
2019年3月6日 · Mice with inactivation of one allele of the Col2a1 gene showed softer articular cartilage, disturbed collagenous network, reduced subchondral …
- 作者: Chengjie Lian, Xudong Wang, Xianjian Qiu, Zizhao Wu, Bo Gao, Lei Liu, Guoyan Liang, Hang Zhou, Xiaom...
- Publish Year: 2019
Immunostaining of Skeletal Tissues - PMC - PubMed Central (PMC)
Immunostaining is the process of identifying proteins in tissue sections by incubating the sample with antibodies specific to the protein of interest, then visualizing the bound antibody using a …
Senolytics cocktail dasatinib and quercetin alleviate chondrocyte ...
2025年1月1日 · Immunohistochemistry (IHC) staining revealed a positive correlation between the ratio of P16 and P21 positive cells and increased OARSI scores, indicating the coexistence of …
Targeting Fascin1 maintains chondrocytes phenotype and ... - Nature
2024年9月4日 · FSCN1 expression is upregulated along with chondrocyte dedifferentiation and OA pathogenesis. a, b SOFG staining and IHC of FSCN1, Osteoarthritis Research Society …
The simultaneous analysis of mesenchymal stem cells and early ...
Bone area measurements and antibody staining were quantified using Nuance Multispectral Imaging System (Calliper) in selected regions differing in degrees of OA bone pathology …
Disruption of Col9a2 expression leads to defects in osteochondral ...
2023年7月1日 · To verify this hypothesis, we then performed TRAP staining and IHC for osteogenesis-related proteins (ALP and Runx2), which demonstrated that Col9a2 −/− mice …
Immunohistochemical analysis of ageing and osteoarthritic …
2010年7月11日 · IHC staining of the tissue sections in group 1B revealed slight to moderate staining for OC, OPN, ON and ALP. The IHC scores were found to be significantly higher …
Autophagy attenuates osteoarthritis in mice by inhibiting …
2023年2月2日 · To investigate the effect of autophagy on chondrocyte metabolism in a DMM-induced OA model, we performed IHC staining for MMP-13 and type II collagen (Col II) in …
