DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.

DAPI is a classic nuclear counterstain for immunofluorescence microscopy, as well as an important component of high-content screening methods requiring cell-based quantitation of DNA content and segmentation (ex/em 341/452 nm).

Step-by-step protocol for the use of DAPI (4′,6-diamidino-2-phenylindole) for nuclear acid (nuclear) staining in fluorescence microscopy.

DAPI (4´, 6-diamidino-2´-phenylindole, dihydrochloride) is a dye that fluoresces blue (455 nm) when bound to double-stranded DNA and excited by exposure to 345 nm light. DAPI binds selectively to the minor groove of adenine-thymine (A-T) regions of DNA, where its fluorescence is approximately 20-fold greater than in the nonbound state.1-3 These

Thermo Scientific™ Pierce DAPI Nuclear Counterstains are high-purity forms of diamidino-2-phenylindole dye for fixed-cell, fluorescent staining of DNA content and nuclei for cellular imaging techniques.

A collection of DAPI Protocols for research, provided by Life Technologies

DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.

DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other structures. When used according to our protocols, DAPI stains nuclei specifically, with little or no cytoplasmic labeling.

DAPI (4′,6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits ~20-fold enhancement of fluorescence upon binding to AT regions of dsDNA. It is excited by the violet (405 nm) laser line and is commonly used as a nuclear counterstain in fluorescence microscopy, flow cytometry, and chromosome staining.

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