When DOPE is combined with DOTAP, it shows high in vitro transfection efficiency, meaning it effectively transfers genes in lab settings. Furthermore, it forms a stable lipid layer and rapidly merges with the outer layer of cell membranes known as the endosomal lipid bilayer.

DOPE (Dioleoylphosphatidylethanolamine) is a neutral helper lipid for cationic liposome and combines with cationic phospholipids to improve transfection efficiency of naked siRNA.

DOPE (Dioleoylphosphatidylethanolamine) is a neutral helper lipid for cationic liposome and combines with cationic phospholipids to improve transfection efficiency of naked siRNA.

1,2-Dioleoyl-sn-glycerophosphoethanolamine is a non-bilayer lipid of the phosphatidylethanolamine class, it adopts non-lamellar reverse hexagonal structures. [1] It forms part of Lipofectamine, a common transfection reagent.

As one of the most commonly used helper lipid used for LNP formulations, DOPE has a small head group, phosphoethanolamine, and two unsaturated oleoyl lipid chains, providing kinked structure and creating a cone geometry that is favorable for the non-bilayer hexagonal (H II) phase crucial for membrane fusion and bilayer disruption process during ...

1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) is a neutral phospholipid and displays sensitivity towards pH change and has been used in the preparation of liposome and giant unilamellar vesicles (GUVs). Phosphatidylethanolamine (PE) is formed primarily in the reaction of CDP-ethanolamine and diacylglycerol.

To consider the effect of lipid acyl chains on their molecular packaging and lipoplex characteristics were also systematically compared using DOPE and DOTAP with their disteroyl equivalents...

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (18:1 (Δ9-Cis) PE (DOPE)) forms a stable lipid layer and rapidly fuses with endosomal lipid bilayer. DOPE in combination with 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) has high in vitro transfection efficiency.

2013年2月1日 · DOPE is responsible for the pH responsive structural transition and the adding of DA can tune the transition pH well-suited to the pH change in the endosomal maturing. This role of DOPE can be generalized for other cationic lipids containing DOPE as a helper lipid.

2023年12月12日 · DOPE promotes fusogenic, inverted hexagonal lipid structures while DOPC promotes more stable laminar structures. Lipopolyplexes containing DOPE showed substantially higher transfection efficiency than those formulated with DOPC, both in vitro and in vivo.