Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity. Its theoretical underpinning originated from L. Onsager's regression hypothesis. The analysis provides kinetic parameters of the physical processes underlying the fluctuations.

2021年4月12日 · Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry.

Chapter 3 provides an overview of the different detection schemes used in FCS before chapter 4 explains different data recording and processing strategies. Chapters 5 and 6 derive the theoretical models, which are required for data tting, for various uorescence.

Fluorescence correlation spectroscopy and the extensions of this technique (FCCS etc.) have been demonstrated as useful techniques in research involving microfluidics and cellular analyses.

2023年7月7日 · Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the quantitative measurement of biomolecular dynamics and interactions. Improvements in biology, computation,...

Fluorescence lifetime correlation spectroscopy (FLCS) uses pulsed lasers, rather than the continuous laser seen in FCS and FCCS. This allows for even more sophisticated analysis, with FLCS eliminating background or spectral crosstalk between fluorophores.

In recent years fluorescence correlation spectroscopy (FCS) has become a routine method for determining diffusion coefficients, chemical rate constants, molecular concentrations, fluorescence brightness, triplet state lifetimes, and other molecular parameters.

Fluorescence correlation spectroscopy (FCS) is one of the many different modes of high-resolution spatial and temporal analysis of extremely low concentrated biomolecules.

Fluorescence correlation spectroscopy (FCS) allows one to measure diffusive properties of fluorescent molecules, and is closely related to FRAP. Instead of measuring time-dependent concentration profiles and modeling the kinetics as continuum diffusion, FCS follows the steady state fluctuations in number density of a very dilute fluorescent ...

Fluorescence correlation spectroscopy (FCS) is a technique in which measurement of fluorescence intensity fluctuations is used to clarify dynamic molecular interactions within a very small space in a solution containing a small number of fluorescent molecules.