Identification of mutations using transposon insertion as a tag has been remarkably efficient when adapted to the rat SSC system. On the contrary, ENU-mediated random mutagenesis usually causes thousands of point mutations throughout the genome and the less sophisticated detection methods [ 13 – 15 ] often leave unknown background mutations ...

In rats, the tfm phenotype results from a base pair replacement in the AR gene (Yarbrough et al., 1990). This mutation yields a normally sized but dysfunctional AR protein. Like tfm mice, the mutant AR is present in all cells in the body throughout life. Tfm male rats have inguinal

In this review, I provide an overview the development of rat genome resources and describe examples of positional cloning in rats in which novel gene functions have been identified. Keywords: disease model, forward genetics, mutation, positional cloning, rat. The laboratory rat (Rattus norvegicus) is a key animal for biomedical research.

KI rats (carrying a Psen1LF mutation) generated to determine potential early pathogenic changes caused by this mutation; survived into adulthood; showing increased levels of Aβ43, a longer and potentially more amyloidogenic Aβ form.

2020年8月2日 · I took into account the rat genes identified by forward genetic methods as well as those inactivated by ENU-mutagenesis and by targeted mutations, the inactivation of which generated a disease or an abnormal phenotype.

As a model for familial amyotrophic lateral sclerosis, rats with a FUS point mutation KI via CRISPR-Cas9 express a physiological level of this mutant, along with cognitive impairment and neuromuscular signs.

2016年10月10日 · In the last two years, advances in transposon- and zinc finger nuclease-mediated gene knockout as well as the establishment and culturing of embryonic and inducible pluripotent stem cells have created new opportunities for rat genetic research.

2008年4月28日 · In the context of significant progress in rat genomic resources over the past decade, we outline achievements in rat gene discovery to date, show how these findings have been translated to...