The transpeptidation reaction occurs in two steps, the first being formation of a covalent enzyme intermediate and the second involving attack of an amine on this intermediate. Here we use defined PG substrates to dissect the individual steps catalyzed by a …

2005年7月6日 · In the present paper, we explore the transpeptidation reactions of this substrate, where the enzyme catalyzes transfer of the glycyl-l-α-amino-ε-pimelyl-d-alanyl moiety to amines. These reactions are believed to occur through capture of an acyl-enzyme intermediate by amines rather than water.

转肽反应 ，英文名transpeptidation ，指肽的一部分和其他肽的一部分或氨基酸的交换转移反应。 如一种肽通过蛋白酶可逆转化为另一种肽，其中氨基酸残基或肽残基从一种氨基化合物转移到另一种氨基化合物。

Transpeptidation reaction is the chemical reaction that forms the peptide cross-links or bonds during the synthesis of peptidoglycan (murein) in a bacterial cell wall. Peptidoglycan or murein is a polysaccharide molecule that consists mainly of alternating repeats of N-acetylglusamine (NAG) and N-acetylmuramic acid (NAM) .

2005年7月6日 · In this paper, we describe the synthesis and kinetic characterization of an analogous thiolester substrate, 3- (N -glycyl- l -cysteinyl)-propanoyl- d -alanyl- d -thiolactate, that the enzyme hydrolyzes and aminolyzes very efficiently (kcat / Km = 1.0 × 10 7 s -1 M -1).

2024年7月1日 · Sortases achieve this by catalyzing transpeptidation reactions in which substrate proteins displaying specific target sequences (e.g., LPXTG) are covalently ligated to cell wall precursors or linked together to form the bacterial pilus [6].

2024年9月30日 · The transpeptidation reaction catalyzed by GGT resulted in a significant taste-enhancing effect of yeast protein-derived peptides, which was confirmed by sensory evaluation, electronic tongue analysis, and molecular docking data.

2022年5月26日 · The transpeptidation reaction of wild-type and mutated Sortase A in the presence of Ca 2+ and its cognate metals, Mg 2+ and Sr 2+, was also investigated to determine the effect of exogenous metal ions on the enzymatic reaction.

The transpeptidation reaction catalyzed by bacterial sortases is a prominent strategy for protein derivatization that possesses these features. Naturally occurring or engineered variants of sortase A from Staphylococcus aureus catalyze a ligation reaction between a five amino acid substrate motif (LPXTG) and oligoglycine nucleophiles.

The products of the reaction are the deacylated tRNA in the P site and the peptidyl-tRNA with the peptide moiety elongated by one aminoacyl residue attached to the tRNA in the A site, as is shown schematically in Fig. 11.2.