Based on the Vaccinia virus Capping Enzyme (VCE), the Vaccinia Capping System provides the necessary components to add 7-methylguanylate cap structures (Cap-0) to the 5´end of RNA (1). In eukaryotes, these terminal cap structures are involved in stabilization (2), transport (3), and translation (4) of mRNAs.

Vaccinia virus capping enzyme was the first capping enzyme to be purified and characterized (Martin et al., 1975). It is a heterodimer of 844-aa and 287-aa polypeptides, encoded by the D1 and D12 genes, respectively, that efficiently executes all three steps in m 7 GpppRNA synthesis.

Vaccinia Capping Enzyme provides all the components necessary to add Cap-0 structures to the 5′ ends of uncapped RNA. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor.

2014年3月4日 · Vaccinia virus capping enzyme is a heterodimer of D1 (844 aa) and D12 (287 aa) polypeptides that executes all three steps in m(7)GpppRNA synthesis. The D1 subunit comprises an N-terminal RNA triphosphatase (TPase)-guanylyltransferase (GTase) module and a C-terminal guanine-N7-methyltransferase (MTas …

2023年8月25日 · Vaccinia virus RNA capping enzyme (VCE) is a heterodimeric enzyme that has been widely used in synthetic mRNA research and manufacturing.

Here, we show that the vaccinia capping enzyme can be used to produce capped RNA in the amounts that are required for large-scale structural studies. We have therefore designed an efficient expression and purification protocol for the vaccinia capping enzyme.

Vaccinia virus capping enzyme enables the synthesis of a wide variety of modified caps. Some of those caps can be expanded their chemical structure through bioconjugation technics. Gene transfer using synthetic mRNA has steadily attracted …

Crystal Structure of Vaccinia Virus Mrna Capping Enzyme Provides Insights Into the Mechanism and Evolution of the Capping Apparatus. Vaccinia virus capping enzyme is a heterodimer of D1 (844 aa) and D12 (287 aa) polypeptides that executes all three steps in m (7)GpppRNA synthesis.

Vaccinia Capping Enzyme is a capping enzyme originating from vaccinia virus and adds a 7-methylguanylate structure (Cap 0) to the 5’ end of RNA (5’-triphosphate RNA). In eukaryotes, cap structures are important for stabilization, nuclear export, and translation of mRNAs.

The vaccinia virus mRNA capping enzyme catalyzes three sequential reactions in the mRNA cap formation pathway(7, 8, 9). Knowledge of the number of active sites catalyzing these reactions and their physical arrangement is fundamental to our …