Microtubules are dynamic components of the cell cytoskeleton that participate in many important cellular processes, including mitosis, cell motility, morphogenesis, and organelle transport (reviewed in Desai and Mitchison, 1997). Microtubules are made up of α/β-tubulin heterodimers that assemble into ∼25 nm cylindrical polymers.

Like actin filaments, microtubules are dynamic structures that undergo continual assembly and disassembly within the cell. They function both to determine cell shape and in a variety of cell movements, including some forms of cell locomotion, the intracellular transport of organelles, and the separation of chromosomes during mitosis.

As central players in the life of the eukaryotic cells, microtubules (MTs) have fascinated cell biologists for decades. The purification of tubulin and the reconstitution of MT polymerization made possible the in vitro study of the assembly properties of these unique polymers.

2015年11月13日 · Microtubules, which are about 25 nanometers in diameter, form part of the cytoskeleton that gives structure and shape to a cell, and also serve as conveyor belts moving other organelles throughout the cytoplasm.

2008年1月1日 · Microtubules are one of the three components of the eukaryotic cytoskeleton and play a central role in many aspects of cell function, including cell division and cell motility. Electron microscopy, of both isolated microtubules and of microtubules in their cellular context, is an essential tool in understanding their structure and function.

Microtubules are filament-shaped, polymeric proteins (~25 nm in diameter) involved in cellular structure and organization. We demonstrate the imaging of individual microtubules using a conventional bright-field microscope, without any additional phase or polarization optics.

Two experimental approaches based on fluorescence microscopy are commonly used to examine organization of microtubules in mammalian tissue culture cells. The first experimental approach involves indirect immunofluorescence staining of chemically fixed cells with tubulin antibody.

Microtubules are one of the three components of the eukaryotic cytoskeleton and play a central role in many aspects of cell function, including cell division and cell motility. Electron microscopy, of both isolated microtubules and of microtubules in their cellular context, is an essential tool in u …

1999年1月1日 · Microtubules are dynamic components of the cell cytoskeleton that participate in many important cellular processes, including mitosis, cell motility, morphogenesis, and organelle transport ...

2010年11月1日 · Individual microtubules have been visualized via fluorescence imaging of dye-labeled tubulin subunits and by video-enhanced, differential interference-contrast microscopy of unlabeled polymers using sensitive CCD cameras.